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After an object is on a layer, it isn t stuck there. You can copy and paste objects to selected layers, or you can move them using the Layers panel. When you move an object to a layer, it s placed in front of all other objects on a layer. To select multiple objects, remember to Shift+click them and then move them in one of the following ways: n Paste objects on a different layer. First cut or copy objects to the Clipboard. Activate the layer on which you want to put the objects and then use the Paste command (choose Edit Paste or press +V or Ctrl+V). This method works well for moving objects that are currently on a variety of layers. n Move objects to a different layer. Click and drag the active objects object icon (to the right of a layer s name) to another layer. When you use this method, it doesn t matter which layer is active. However, you can t move objects from multiple layers to a single layer using this method. (If you select multiple objects that reside on different layers, dragging the icon moves only objects that reside on the first layer on which you selected an object.) n Move objects to a hidden or locked layer. Press or Ctrl while you click and drag the active objects object icon. n Copy rather than move objects to a different layer. Press Option or Alt while you click and drag the active objects object icon. n Copy objects to a hidden or locked layer. Press Option+ or Ctrl+Alt while you drag the active objects object icon.

enough screen space, you can leave the Track Info pane open if you prefer.)

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embryonic-like stem cells (CBEs) [Forraz et al., 2004; McGuckin et al., 2004]. The CBEs had formed embryoid body-like colonies, which were immunoreactive for primitive human embryonic stem cell-speci c genes [Gerrard et al., 2005; Martin et al., 2004]. Thus McGuckin et al. [2004] suggested that CBEs have the capacity to differentiate into neuronal, hepatic, and pancreatic cells, bone, fat, skeletal muscle, and blood vessels. 7.1.2.5. Cord Blood-Derived Multipotent Progenitor Cells (CB-MPCs). Our group has studied whether other stem cells such as MSCs are present in fresh or cryopreserved UCB. We have also isolated a novel cell line from a population of stem cells found in the human UCB, which had characteristics different from those of HSCs and MSCs. Seeded UCB-MNCs formed adherent colonies of cells in optimized culture conditions. Over a 3- to 4-week culture period, the colonies gradually developed into adherent monolayer cells, which exhibited homogeneous broblast-like morphology and immunophenotypes and were highly proliferative; these will be referred to here as cord blood-derived multipotent progenitor cells (CB-MPCs). CB-MPCs had the capacity to differentiate into various spectra of cell types, including osteoblast, endothelial, hepatic, and neuronal cells. Neuronal-differentiated cells expressed cell type-speci c markers, such as tyrosine hydroxylase (dopaminergic neurons), acetylcholinesterase (cholinergic neurons), and glutamate decarboxylase (GABAergic neurons), suggesting that CB-MPCs differentiate into functionally speci c neurons [unpublished results].

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