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Nonsterile Trypan Blue Ice bath Protocol (a) Weigh the container (500 ml Erlenmeyer ask) to be used to hold the sample (b) Excise the cartilage from the joint surface with a #10 or #15 scalpel blade and place the explants into dissection medium on ice Approximately 7 g of cartilage can be excised from the articular metacarpophalangeal joint surfaces of a yearling (15 18 month old) steer (c) After excision of the articular cartilage, wash the explants several times in dissection medium Repeat the washes until the supernatant remains clear after agitation (d) At the completion of the wash steps, remove as much liquid as possible and reweigh the container The difference between the rst and second weights gives a reasonable estimate of the weight of cartilage collected (e) Transfer the cartilage into a sterile 10 cm Petri dish and immerse in dissection medium.

(f) Dice the samples into approximately 2 mm-thick slices (g) Transfer the diced cartilage into an Erlenmeyer ask (h) Predigest the explants in trypsin-EDTA (10 ml/g) containing penicillin-streptomycin in a shaking incubator at 37 C for 1 h (i) Rinse the explants in dissection medium, then add the digestion medium for bovine articular cartilage Replace the ask in a shaking incubator at 37 C overnight (j) After overnight digestion, lter the digestion medium through 40 m lters (k) Pellet the isolated cells by centrifugation at 250 g for 10 min (l) Aspirate the supernatant and resuspend the cells in 10 volumes of Ham s F-12 medium (m) After 2 3 rounds of centrifugation and resuspension, cell number can be determined with a hemocytometer and assessed for viability by Trypan Blue exclusion Approximately 1 106 chondrocytes (969 105 1.

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writing can fully interoperate with BOOTP servers and clients. Terms used in the DHCP protocol are as follows: DHCP Client: A host that is requesting configuration information. DHCP Server: A DHCP hosts that supplies configuration parameters to a requesting host. BOOTP Relay Agent: The protocol that allows BOOTP and DHCP packets to traverse a router. Binding: Configuration parameters, including an IP address, that are bound to a host.

85 105 , n = 8) can be isolated from each gram of articular cartilage obtained from the metacarpophalangeal joints of yearling steers..

where the objective is to maximize the total end-to-end transmission rate. This end-to-end transmission rate is a minimum of transmission rate among all hops. We solve this optimization problem by enumeration. Note that the computational complexity of solving this optimization is substantially large and also the subchannel information for all the mesh routers along the route to the destination would be required. Therefore, a distributed implementation of this global optimization might not be possible. For numerical results, we consider a chain topology with three hops (i.e., BS4 to BS-6 in Fig. 21.8). The end-to-end transmission rate per connection is shown in Fig. 21.11. As expected, with the objective de ned in (21.19) the endto-end transmission rate achieved via global optimization is only slightly larger (i.e., about 10 18%) than that for the proposed subchannel allocation algorithm, which is performed in a distributed manner. Since the objective in global optimization is to maximize the transmission rate at the bottleneck BS (i.e., BS with minimum transmission rate), with the global information of all BSs, endto-end transmission rate for a connection obtained from this optimization formulation is larger than that from the proposed distributed algorithm. However, note that the proposed distributed subchannel allocation approach does not require global information of all the mesh routers along the route to the destination, and it incurs much lower computational complexity. 21.7.2.4 Optimization of the Resource Reservation Threshold Performance of the Bargaining Game. To evaluate the performance of the bargaining game formulation for optimizing the resource reservation threshold for subchannel allocation, we consider 15 connections (including both local and relay connections) at a particular BS. While the average SNR for

where ci , c j are two code sequences that correspond to the message sequences mi = m j . The minimum free distance is de ned as the minimum distance between any two code sequences of the convolutional code. Assuming that the convolutional code is linear and that transmission over the channel makes use of a geometrically uniform signal set [17 26], the calculation of the minimum distance between any two code sequences is the same as determining the weight of the sum of these two code sequences as df = min w(ci c j ) : mi = m j = min {w(c) : mi = 0} (34)

Fetal bovine serum (FBS) 10% Dulbecco s modi ed Eagle medium (DMEM) 90% Filter the solution, using a 0.22- m lter bottle.

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